The ex-villain Gru is raising his daughters Margo, Edith and Agnes and researching a legitimate jelly and jam business with Dr. Nefarion and the minions. Meanwhile, a laboratory in Arctic is stolen by a powerful aircraft using magnetism. The Anti-Villain league (AVL) agent Lucy Wilde recruits Gru to help her agency discover who has stolen the laboratory and its research. They know that the thief has a store in a mall, and Gru suspects that the owner of the Mexican restaurant Salsa & Salsa, Eduardo Perez, may be the villain El Macho that is presumed dead.
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